Electrochemical detection of interaction between daunorubicin and DNA by hybrid nanoflowers modified graphite electrodes

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Makaleler - Electrochemical detection of interaction between daunorubicin and DNA by hybrid nanoflowers modified graphite electrodes Update: 2022-01-13 08:25:19

Electrochemical detection of interaction between daunorubicin and DNA by hybrid nanoflowers modified graphite electrodes

Mukerrem Findik, Haluk Bingol, ArzumErdem
  • 2021-01-14
  • Sensors and Actuators B: Chemical
Summary
In this study, two types of hybrid nanoflowers (NFs) modified disposable electrodes were developed to investigate the electrochemical behavior of anticancer drug-DNA interaction. Biocompatible hybrid NFs were synthesized by amino acids (L-gluta.. Full Article

Abstract

In this study, two types of hybrid nanoflowers (NFs) modified disposable electrodes were developed to investigate the electrochemical behavior of anticancer drug-DNA interaction. Biocompatible hybrid NFs were synthesized by amino acids (L-glutamic acid and l-cysteine) via a simple, rapid and cost-effective methods. Structural characterizations of the synthesized hybrid NFs were performed with Attenuated Total Reflection-Fourier Transform Infrared (ATR-FTIR), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS), while morphological characterizations were performed using scanning electron microscopy (SEM) and energy diffuser X-ray (EDX) spectroscopy techniques. As the sensing platform in electrochemical studies, pencil graphite electrode (PGE) has been used because of its advantages such as low cost, easy-to-handle, portable and disposable. After PGEs modified with NFs were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques, differential pulse voltammetry (DPV) technique was used in the determination of calf thymus double stranded DNA (ctdsDNA) and also daunorubicin (DNR) as well as DNA-DNR interaction. The detection limits as a result of ctdsDNA determination were found to be 0.93 μg/mL by using l-cysteine NFs modified PGE (c-NFs-PGE) and 2.37 μg/mL by using l-glutamic acid modified PGE (ga-NFs-PGE). The detection limits obtained for the determination of DNR were found as 2.93 μM and 2.06 μM with c-NFs-PGE and ga-NFs-PGE, respectively. The optimum interaction time between DNR and ctdsDNA was determined by c-NFs-PGE, which showed higher sensitivity performance against oxidation of guanine signals.

Keywords

Hybrid nanoflowers
Electrochemical sensors
Anticancer drug-DNA interaction
Daunorubicin
Pencil graphite electrode

Mukerrem Findik received her degree in Chemistry at the Selcuk University (Konya, Turkey) in 2006. She defended her Master Degree (2009) and PhD (2014) in Analytical Chemistry at the Selcuk University. She was working as postdoctoral researcher at Prof. Dr. Arzum Erdem’s lab in Analytical Chemistry Department in the Faculty of Pharmacy of Ege University (İzmir, Turkey) from October 2018 to March 2020. Her work focused on DNA determination and DNA-anticancer drug interaction with pencil graphite electrode developed with organic-inorganic hybrid nanoflowers.

Haluk Bingol obtained his PhD in Chemistry from Selcuk University in 2007. He is a Professor in the Chemistry Department at the Necmettin Erbakan University (NEU) in Turkey. Currently, Prof. Bingol is also director of the Science and Technology Research and Application Center (BITAM) at NEU. His research activities include the preparation of new nanomaterials such as graphene-based materials, nanoparticles, quantum dots and nanofiber for their application in sensors. He is author of more than 50 publications in the sensor and nanotechnology field in refereed scholarly journals.

Arzum Erdem is a Professor at the Analytical Chemistry Department in the Faculty of Pharmacy of Ege University in Turkey. She received her PhD in Analytical Chemistry from Ege University in 2000. Dr. Erdem was awarded by the Turkish Academy of Sciences (TUBA) as the one of highly skilled young scientists selected in 2001, and she also received Juniour Science Award in 2006 and Science Award in 2015 given by The Scientific and Technological Research Council of Turkey (TUBITAK). She was elected as the Associate member of TUBA in 2007, and elected as the Principal member of TUBA in 2016. Arzum Erdem was elected as the Special Committee member of Association of Academies and Societies of Sciences in Asia (AASSA)- Women In Science and Engineering (WISE) for the period of 2017-2019 and 2019-2021. She was elected as a fellow of Royal Society of Chemistry (FRSC) in December 2017. Prof. Dr. Arzum Erdem has initiated many international collaborative research on development and applications of electrochemical (bio)sensors based on drug, enzyme and nucleic acids. Her recent research is centred on the development of novel transducers and chemical and biological recognition systems by using different nanomaterials (e.g, magnetic nanoparticles, carbon nanotubes, graphene, dendrimers, gold and silver nanoparticles, nanowires, nanorods etc.) designed for electrochemical sensing of nucleic acid (DNA, RNA) hybridization, and also the specific interactions between drug and DNA, or protein and DNA, aptamer-protein and also the development of integrated analytical systems for environmental, industry and biomedical monitoring.

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